Workforce is bidding to become the first Derby winner to go on to beat his elders in the King George VI And Queen Elizabeth Stakes at Ascot since Galileo in 2001.
Owner Khalid Abdullah has seen his colours carried to victory just once in the famous race - by the brilliant Dancing Brave in 1986.
Dancing Brave finished second at Epsom, while Workforce was an impressive seven-length winner, and the King's Best colt has had a smooth preparation for the Betfair-sponsored test.
"It's going to be a really exciting day. He's had a straightforward preparation, he has worked nicely and, most importantly, without any interruptions. The line-up is what most neutrals would want to see for the King George," said Abdullah's racing manager Teddy Grimthorpe.
The owner also runs outsider Confront as a pacemaker for his Classic hero.
Mick Channon's luckless seven-year-old Youmzain has his third crack at the race, having finished second to Dylan Thomas in 2007 and grabbed third behind Duke Of Marmalade a year later.
"I don't know how he's going to run - he's Youmzain isn't he!" quipped Channon.
"I've been very pleased with him and we're pleased with the way his preparation has gone. He ran a blinder in France last time. He's a year older and probably a year wiser, but I'd be very hopeful."
John Gosden's three-time Group One winner Dar Re Mi has been ruled out of the race after suffering a foot injury.
The trainer's racing secretary Peter Shoemark said: "She has bruised her foot and unfortunately that means she will be unable to run. It's just unfortunate it has come so close to the race, but she will be absolutely fine. The Yorkshire Oaks could be her next objective."
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