Green Destiny, one of the leading contenders for the Royal Hunt Cup, is set to miss the mile handicap.
Trainer William Haggas instead plans to run the four-year-old in the Listed Wolferton Handicap on Friday.
"We have decided to withdraw Green Destiny as I consider the ground could be too fast for him and he will not be as effective over that distance on fastish ground," said Haggas.
"His owner, Saleh Al Homaizi, is very conscious of the fact that the horse is and has been one of the favourites for the race for a long time.
"But he has accepted my view that running him over 10 furlongs in the Wolferton Handicap on Friday is more suitable for the horse, especially with rain forecast. He will therefore be declared for Friday's race."
Meanwhile Roger Varian is hoping Eton Forever, who was only fourth in Listed company at Ascot last time, can step up on that performance.
The trainer said: "He was a little bit flat at Ascot, he wasn't quite 100% when he got home. It wasn't a bad run, but perhaps not quite up to his true ability. He's very well, but it's a very good race and it will be a tough task off that mark.
"You don't get many winners of it rated over 100, but if you are going to run in handicaps you may as well run in a big one, so we'll see how he gets on.
"He's drawn 15, which is right in the middle. I'd almost rather be on one side or the other, then you don't have a decision to make.
"Being in the middle you either go up one side or the other and that means you're not going in a straight line, so I'd have rather have been one side or the other."
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